Noboru MIZUSHIMA B6;129-Atg5<tm1Nmz> B6;129-Atg5<tm1Nmz> true <a href='https://brc.riken.jp/mus/pcr02231'>Genotyping protocol -PCR-</a> Heterozygote x Wild-type [C57BL/6JJmsSlc] Heterozygote x Wild-type [C57BL/6JJmsSlc] Atg5 knockout mice. Exons 1 and 2 of Atg5 were replaced with a neomycin resistance gene. Homozygous mutant mice die during the early neonatal period due to a lack of nutrients. Autophagy is an intracellular degradation process by an autophagosome, which contains a portion of cytoplasm and subsequently degrades upon fusion with a lysosome. Autophagy is considered to be important for the cellular response to starvation and the normal turnover of cytoplasmic components as well. Atg5, autophagy-related 5 is essential for autophagosome formation. Conditional mice (floxed allele) are also available: B6.129S-Atg5<tm1Myok> (RBRC02975). herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Atg5 genomic DNA 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Nature, 432, 1032-1036 (2004).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>非営利団体による学術研究に限る。当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に 寄託者との間で、別途使用許諾に係る契約の締結が必要である。利用者が本件リソースを使用してえられた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 Tokyo Med. Dent. Univ. R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] Developed by Masahiko Hatano and Takeshi Tokuhisa, Graduate School of Medicine, Chiba University, and Noboru Mizushima, National Institute for Basic Biology in 2003. The knockout construct was electoroporated into R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+>. The mutant mice were backcrossed to C57BL/6J. Atg5, autophagy-related 5 (yeast)のノックアウトマウス。エクソン1と2をneoカセットで置換。ホモマウスは全身でオートファジーが不能となり、ホモは出生後12時間以内に死亡する。コンディショナルKOマウス (floxedアレル) もあり (RBRC02975 B6.129S-Atg5<tm1Myok>) 。, <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/jul_2009_mm" target="_blank">Mouse of the Month Jul 2009</A><br><a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/02231_GB.pdf">Genetic Background</a> The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 432, 1032-1036 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization.　Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution.　The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://molbiolut.jp/en/" target="_blank">Lab HP</A> Cell Biology Research RBRC02231 千葉大学大学院医学研究院・幡野雅彦先生、徳久剛史先生、および基礎生物学研究所・水島　昇先生（2003）。R1 ES細胞を用いて作出。C57BL/6Jへ戻し交配された。 Atg5, autophagy-related 5 (yeast)のノックアウトマウス。エクソン1と2をneoカセットで置換。ホモマウスは全身でオートファジーが不能となり、ホモは出生後12時間以内に死亡する。コンディショナルKOマウス (floxedアレル) もあり (RBRC02975 B6.129S-Atg5<tm1Myok>) 。 C（3〜6か月） 水島　昇 C (3-6 months) 国立大学法人東京医科歯科大学